Last edited by Tazil
Monday, August 3, 2020 | History

2 edition of Quantitation of the unstable expression of a metastatic phenotype in murine tumor cells. found in the catalog.

Quantitation of the unstable expression of a metastatic phenotype in murine tumor cells.

Scott Donald Young

Quantitation of the unstable expression of a metastatic phenotype in murine tumor cells.

by Scott Donald Young

  • 216 Want to read
  • 12 Currently reading

Published .
Written in English


The Physical Object
Pagination121 leaves
Number of Pages121
ID Numbers
Open LibraryOL18882750M

Histones H and H were observed in MDA-MB- metastatic breast cells, whereas an additional histone variant, histone H, was identified only in non-neoplastic MCFA cells.   Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO) Citing These Resources Funding Information.

To characterize the tumor biology of breast cancer metastases, we assessed a set of seven biologically relevant gene expression modules in our metastatic samples [].For visualization purposes, we selected those genes positively correlated with each module from our samples and carried out hierarchical clustering (Figure 1A and B). All gene modules (AURKA—proliferation, . Growth arrest–specific 1 (Gas1) plays a critical role in growth suppression. Previous study indicated that Gas1 was closely associated with survival in patients with colorectal cancer; however, the underlying molecular mechanism remains unclear. In this study, we sought to determine the role of Gas1 in tumorigenesis and metastasis, and elucidate the possible mechanism.

In-transit metastatic melanoma, which typically presents as multifocal lesions, provides a unique setting to evaluate the utility of gene signatures for defining optimal regional therapeutic strategies and assessing the efficacy of treatment. The goal of this study was to determine whether a single multifocal lesion is representative of residual tumor burden in terms of gene expression.   Moreover, MTA2 is significantly associated with tumor grade (P tumor (P expression in oral cancer cells was markedly higher than that in normal oral cells. Cell proliferation and cell cycle were not significantly changed in the cells inhibited by MTA2.


Share this book
You might also like
Shark attack

Shark attack

Montale and Dante.

Montale and Dante.

Managing volunteers for results

Managing volunteers for results

The past and future of money

The past and future of money

Survey of foreign, English-language literary quarterlies available for exchange

Survey of foreign, English-language literary quarterlies available for exchange

108th Congress: The Senate of The United States, Committee and Subcommittee Assignments, S. Pub. 108-5, March 31, 2003, *

108th Congress: The Senate of The United States, Committee and Subcommittee Assignments, S. Pub. 108-5, March 31, 2003, *

Parasites of the human heart

Parasites of the human heart

Nato And The European Union

Nato And The European Union

Epidemiologic Research Solution Manu1290

Epidemiologic Research Solution Manu1290

Bedford County Tennessee, Wills & Vital Records from Newspapers

Bedford County Tennessee, Wills & Vital Records from Newspapers

Attitude

Attitude

UNDERSTANDING the freight business.

UNDERSTANDING the freight business.

Migrant and seasonal farmworkers

Migrant and seasonal farmworkers

Memorandum by the Evangelical Church in Germany on the question of war crimes trials before American military courts.

Memorandum by the Evangelical Church in Germany on the question of war crimes trials before American military courts.

A little touch of Harry

A little touch of Harry

Support for East European Democracy (SEED) Act of 1989

Support for East European Democracy (SEED) Act of 1989

Quantitation of the unstable expression of a metastatic phenotype in murine tumor cells by Scott Donald Young Download PDF EPUB FB2

INTRODUCTION. The incidence of malignant melanoma is increasing faster than that of other solid tumors [].The 5-year survival rate is less than 20% for distant-stage metastatic malignant melanoma [], indicating that metastasis is the main factor in a poor addition, the aggressive characteristics of malignant melanoma results in an innate and acquired resistance to Cited by: One relevant aspect of the phenotype of transformed cells is their reduced adhesion to solid substrates; this phenomenon is thought to reflect the invasive and metastatic potential of tumor cells.

Since the adhesion of the cells to substrata is mediated by molecules of the extracellular matrix, the expresssion of extracellular matrix receptors Cited by: Abstract. The acquisition of the ability to metastasize is the ultimate and most deadly level of the tumorigenic progression.

Metastatic cells are able to dissociate from the primary tumor, invade through the surrounding tissue and endothelial layer into the bloodstream, migrate through the bloodstream to the site of metastasis and then reinvade and proliferate to generate a new tumor lesion Author: I.

Nabi, A. Raz. Progression toward metastatic phenotype with loss of growth‐inhibiting tumor‐cell/cell interactions in vivo. Yasutaka Takeda. of autonomous sub line tumor cells to growth‐inhibitory regulation from the parental hormone‐dependent tumor cells correlated well with growth morphology within collagen gels and meta‐static ability, but Cited by: 3.

Because the original breast tumor from which MDA-MB was derived was composed of a mixture of metastatic, less metastatic, and nonmetastatic tumor cell populations, we also compared the expression profiles of metastases with that of sister (isogenic) cells in nonmetastatic NM-2C5 primary tumors, growing in a different set of mice of the same Cited by:   Cell Biology International Reports.

Vol. August PRODUCTION OF METASTATIC PHENOTYPE WITH DNA FROM METASTATIC CELLS BUT NOT WITH ONCOGENIC DNA Susan Jamieaon, Roger Barraclough and Philip S.

Rudland Cancer and Polio Research Fund Laboratories, Biochemistry Department, Liverpool University, Liverpool L69 3BX, U.K.

*Beatson. Single-step mutants were isolated from the murine metastatic MDAY-D2 cell line after selection in toxic concentrations of wheat-germ agglutinin. They were partially characterized by measuring their relative level of resistance to WGA, PHA, Con A, RIC, and LCA (Lec phenotype), and by comparing their karyotype and their ability to produce metastases upon transplantation into syngeneic DBA/2 mice.

The expression of Fyb mRNA was also lower in Sq, −2, −3, and −11 cells compared with L cells, including L,6–8 and 6–9 cells. The expression of Slc16a13, Temem and Slc44a3 mRNA was almost undetectable in the three Sq sub-clones and cells, whereas the expression was high overall in all 5 L cells.

The problem how tumor cells get the metastatic ability is still a hot debate. Based on the premise that the default state of normal cells is quiescent rather than mobile, the classic progression and early metastasis model suggested that tumor metastasis should be an acquired trait contributed by the late or early gene mutations during carcinogenesis.

Signal Transduction Acquisition of the Metastatic Phenotype Is Accompanied by H 2O 2-Dependent Activation of the pCas Signaling Complex Nadine Hempel 1, Toni R.

Bartling, Badar Mian2, and J. Andres Melendez Abstract Reactive oxygen species (ROS) have emerged as cellular signaling molecules and are implicated in metastatic.

Method and Result: In-depth genetic analysis of tumor cells, especially with advances in the next-generation sequencing, have revealed insights of the genotypes of metastatic tumor cells. Also, studies have shown that the cancer stem cell (CSC) and epithelial to mesenchymal transition (EMT) phenotypes are associated with the metastatic cascade.

In addition, only 9 of 15 5-aza-dCyd-treated clones produced tumor takes because of the ability of 5-Aza-dCyd to engender Imm+ variants in CBA-SP1 cells. Lung metastases obtained after the injection of uncloned cells retained their metastatic phenotype for three generations, indicating that the phenotypic change was a heritable characteristic.

Metastasis is commonly believed to result from the clonal selection of a few rare cells in a tumor population ().An alternative mechanistic model for metastasis was suggested on the basis of DNA microarray studies implying that the proclivity for metastasis is hardwired in progenitor cells ().One study showed that gene expression profiles in primary breast tumors are strikingly similar.

Table 1. Verification of the in vivo metastatic potential of the rat cell lines used. A total of 5 × 10 5 viable cells in PBS were injected s.c. into the flank of each animal. Three to nine animals were used for each cell line, and the animals were maintained until the animals became moribund, or the primary tumor had reached the legal limit as set by German animal protection regulations.

Nevertheless, the influence of the surroundings on the definition of the metastatic cell phenotype is of outstanding relevance today (3). The modern understanding of the heterogeneity of tumor cells, including the metastatic phenotype, contemplates a rainbow of intrinsic and extrinsic inducing factors (4), among them, local hypoxia has rightly.

Basal protein expression was correlated with in vitro response to the MEK inhibitor, selumetinib. Protein expression in a cohort of 32 drug naïve BRAF/NRAS metastatic melanoma specimens was examined. Residual tumor cells remaining after treatment (R x) may rely on functions that had been previously selected during the micrometastatic state.

Individual metastatic traits, e.g., the expression of a metastasis gene, mediate particular functions that slightly increase the probability that a cancer cell will perform a particular step of. However, she added, metastatic cancer cells do have specific characteristics that differentiate them from normal cells and the other cancer cells in the primary tumor.

Among these characteristics are PNCs. In addition to being ubiquitous in metastatic cancer cells, PNCs also have been linked with poor survival in several different cancer types. Gene expression profiling of metastatic brain tumors from primary lung adenocarcinoma, using a 17k-expression array, revealed that genes were consistently altered.

Further functional classification placed the genes into seven categories: cell cycle and DNA damage repair, apoptosis, signal transduction molecules, transcription factors. Ostapoff, K. et al. Neutralizing murine TGFβ R2 promotes a differentiated tumor cell phenotype and inhibits pancreatic cancer metastasis.

Cancer Res. 74, – (). MetC showed low expression of luminal cell markers, but was enriched for some transcripts indicating a basal cell phenotype, that is, CEBPB and GSTP1. Other basal cell markers like p63 and CK5 were low in all cases. Expression levels of luminal cell markers. Although the tumorigenic phenotype can be transferred by transfecting DNA from a variety of human tumours into 3T3 mouse fibroblasts, the ability to transfer the metastatic phenotype in a similar manner in syngeneic animals is largely unknown.It is highly expressed in various metastatic tumor cells and its elevated expression is associated with the poor prognosis of different types of human cancers [2] [3][4][5][6][7].

The involvement.